UV-Vis Spectrophotometer

    Kindly download Analysis Request Form and Safety Data sheet and submit the hardcopy of filled sheet along with your sample

  Facility Details  

Make : Agilent Technologies
Model : Carry 100 UV-Vis
Specification : Monochromator. Czerny-Turner 0.278 m.
  Grating 30 x 35 mm, 1200 lines/mm, blaze angle 8.6° at 240 nm
  Beam splitting system. Chopper (30+ Hz)
  Detectors. R928 PMT
  UV-Vis limiting resolution. ≤ 0.189 nm
  Wavelength range. 190-900 nm
  Wavelength accuracy: At 656.1 nm (± 0.02 nm)
           : At 486.0 nm(±0.4nm)

     Qualitative and quantitative Analysis
     Protein/RNA/DNA quantification
     Metal Concentration
     Lab no 413B, First Floor,
     CRNTS/SAIF, IIT Bombay


     1. Integrating Sphere- Thin film and polymer samples/Powder samples/Liquid samples
     2. Two cell holder - Liquid samples
     3. Standard- Reference material (BaSO4)
     The Cary 100 is a cost-effective UV-visible spectrophotometer with a versatile set of accessories for routine laboratory work. It is controlled by the Cary WinUV software,
     a Windows-based software featuring an easy-to-use modular design. The instrument is shipped with liquid sample holders and can be fitted with a wide range of accessories
     to provide extra capabilities. The instrument is popular in university teaching laboratories.

   Working Principle   

The Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. Spectroscopy is based on the interaction between light and matter. When the matter absorbs the light, it undergoes excitation and de-excitation, resulting in the production of a spectrum.
When matter absorbs ultraviolet radiation, the electrons present in it undergo excitation. This causes them to jump from a ground state (an energy state with a relatively small amount of energy associated with it) to an excited state (an energy state with a relatively large amount of energy associated with it). It is important to note that the difference in the energies of the ground state and the excited state of the electron is always equal to the amount of ultraviolet radiation or visible radiation absorbed by it.

   User Instructions   

     1. Provide solid samples in the powder form. Minimum required amount is approximately 2-3 gm.
     2. Samples may be in liquid form. The required minimum amount is 2-3 ml.
     3. Samples can be submitted as thin film, Solid pellets, powder etc.
     4. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there sample being given. Samples should not be toxic or hazardous.
      Samples will not be accepted unless MSDS is provided.

  Contact Us   
      CRNTS/SAIF IIT Bombay
      Contact: 022-2159 6869
      Email Id : mngandhi@iitb.ac.in,pallaviag@iitb.ac.in,

 UV-Visible spectroscopy system charges includes GST 

IndustryUniversityNational Lab/R&D'sIIT Bombay Users
UV- Vis spectroscopy 1180/- 236/-531/-100/-Per Sample